Download HIV Rapid Screen Test instructions (PDF)

WHY AND WHO SHOULD TEST FOR HIV?

HIV is a virus that causes AIDS (Acquired Immune Deficiency Syndrome). Anyone can be at risk. Most infected people do not know their HIV status as they look and feel well. The most common routes of HIV transmission are:

  • Unprotected sexual contact with infected person(s)
  • Repeated use of needles in connection with drugs
  • Use of unsafe blood products or transfusion with infected blood
  • Transfer of infection from an HIV positive mother to her child
  • Intravenous drug users
  • Health care workers who take blood samples

WHAT IS THE HIV 1 & 2 RAPID SCREEN TEST USED FOR?

The HIV 1 & 2 Rapid Screen Test will give you a quick and accurate test result within 15 minutes from the privacy of your own environment.

It is an aid only to identify infected individuals. According to studies, the typical period of time between the first contact and a positive test indication is about 90 days. This is the time that the body requires to create antibodies against the HIV virus.

WHEN SHOULD I TAKE THE TEST?

If you have reason to suspect a possible infection of yourself or your sexual partner(s), it is wise to get tested. We advise you to wait 3 months before you take a test because, even though, most people will manifest antibody levels within 30 days, a significant percentage of them do not show these anti-bodies until about 90 days. The National CDC has said that in rare cases, it may even take up to six months for one to get an HIV positive result. We recommend you to do 2 tests 30 days apart.

WHAT IF I TEST POSITIVE FOR HIV?

If you get an HIV positive result, consult your doctor immediately.

CONTENTS OF HIV 1 & 2 RAPID SCREEN TEST

Each Kit Contains:

  • HIV 1 & 2 test card, individually foil pouched with a desiccant (silicon gel)
  • Plastic dropper
  • Sample diluent
  • Safety lancet
  • Alcohol swab
  • Package insert

Material Required But Not Provided:

Timer

STORAGE AND STABILITY

The kit must be stored between 2-30OC.

DISCLAIMER

When performing the HIV test, the steps involved must be followed closely. If you think you were exposed to infected blood, and you get an HIV negative result, the test should be repeated in 90 days. It is imperative that a positive HIV test result is followed by a Western Blot or PCR test performed by a doctor or clinic to confirm if you are indeed HIV positive. Since NO test, nor test kit is infallible, it is best to confirm test results at a licensed medical testing facility. THIS TEST CAN ONLY BE USED ONCE.

Do not open pouch until you are ready to test the sample.

ASSAY PROCEDURES FOR FINGER BLOOD

  1. Bring the HIV test card, sample diluent, alcohol swab, safety lancet, plastic dropper to room temperature.
  2. Take out the test card from the sealed pouch.
  3. To perform the HIV test please follow the steps from picture 1 to picture 8.

ASSAY PROCEDURES AT CLINICS

  1. Bring all reagents and specimens to room temperature.
  2. Remove the test card from the foil pouch and place on a clean dry surface.
  3. Identify the test card for each specimen or control.
  4. For whole blood/serum/plasma testing: Dispense one drop (30μl) of sample or control into the sample well on the card using the plastic dropper provided, then add one drop (50μl) of sample diluent into the same well.
  5. Interpret test results at 15 minutes. A positive result may be interpreted early, however, read any negative at 15 minutes to ensure sample is negative and not a low concentration of the anti-HIV antibody. Do not interpret the result after 20 minutes. 

It is recommended to run a known positive control and negative control in each performance to ensure the assay procedure.

READING THE TEST RESULTS

  1. Positive: Both purplish read test band and purplish read control band appear on the membrane. The lower the antibody concentration, the weaker the test band.
  2. Negative: Only the purplish red control band appears on the membrane. The absence of a test band indicates a negative result.
  3. Invalid: There should always be a purplish red control band in the control region regardless of the test result. If control band is not seen, the test is considered invalid. Repeat the test using a new test device.

Note: It is normal to have a slightly lightened control band with very strong positive samples as long as it is distinctly visible.

BIBLIOGRAPHY

  1. Guyader,M., Emerman, M., Sonigo P., et al. Genome organization and transactivation of the human immunodeficiency virus type 2. Nature, 326:662-669. 1987.
  2. BIattner, W., Gallo, R.C. and Temin. H.M. HIV causes AIDs. Science, 241∶ 515, 1988.
  3. Curran, J.W.,Morgan. WM., Hardy, A. M., et al. The epidemioIogy of AIDS: Current status and future prospects. Science 229∶ 1352-1357, 1985.
  4. M.G., PopovIc. M.,Bruch,L ., Schupback, J., and Gallo, R.C. Antibodies reactive with human T-lymphotropic retroviruses (HTLV-III) in the serum of patients with AIDs. Science.224∶ 506-508. 1984.
  5. Weber, J.N, Weiss, RA., Roberts, C., et al. Human immunodeficiency virus infection in two cohorts of homosexual men∶ Neutralising sera and association of anti-gag antibody with prognosis. Lancet 1∶ 119- 124. 1987.
  6. Clavel, F., Guetard. D., Brun-Vezinet,F., et al. Isolation of a new human retrovirus from West African patient with AIDs. Science 233∶ 343- 346.1986.

THIS TEST KIT IS INCLUDED IN THESE PRODUCTS